The South Atlantic Meridional Overturning Circulation Basin-wide Array (SAMBA) Monitoring Line in the South-East Atlantic Ocean was conducted on the Algoa Voyage 275 from 13 September to 7 October 2021. The cruise operated from Cape Town towards Slangkop and westwards to 0° E, in an easterly direction in the South Atlantic Ocean along the SAMBA Monitoring Line (34.5°S). The objectives of the cruise were to recover, service and deploy 10 CPIES/PIES on the SAMBA transect; attempt to locate and recover DFFE current meter mooring (M7), that could not be recovered during 2020 cruise; undertake full depth Conductivity, Temperature, Depth (CTD) casts along the SAMBA transect (37 stations) using containerised winch system; undertake chlorophyll, oxygen, nutrient and Dissolved Inorganic Carbon (DIC) sampling at CTD stations and underway along SAMBA transect; collect under-way Thermosalinograph (TSG); Surface Photosynthetically Active Radiation (SPAR) and Ship-mounted Acoustic Doppler...