Dissolved inorganic macronutrients nitrate (NO3), nitrite (NO2), silicate (PO4) and phosphate (PO4) in the upper waters of the ocean. The availability of these macronutrients limits and regulates the production of organic matter during photosynthesis. Therefore, phytoplankton uptake of the macronutrients plays a necessary role concerning biogeochemical cycling of carbon around Gough Island and Southern Ocean. Here we present macronutrients data collected along the upstream, near island and downstream of the Gough Island (39.8 °S, 9.5 °W and 40.8 °S, 10.4 °W) during the yearly logistical voyage of the Department of Forestry, Fisheries and the Environment (DFFE) from the 5 September to 7 October 2024. We sampled 10 stations which were grouped into three categories: upstream stations, near island stations and downstream stations. Seawater samples were analysed for macronutrients concentrations within three months of collection, with detection limits of 0.03 μM for nitrate + nitrite,...